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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circularly polarized luminescence. As drawn in Fig - https://www.cybo.com/US-biz/on-line-instrument-systems-olis-inc. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this might be because the H4B extreme serve as an electron sink in a later point in the response, so regarding permit NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.